Screening for metronidazole-resistance associated gene fragments of H pylori by suppression subtractive hybridization.

ABSTRACT:

Aim

To screen for metronidazole (MTZ)-resistance associated gene fragments of H pylori by suppression subtractive hybridization (SSH).

Methods

Five MTZ-resistant (tester, T) and 1 MTZ-susceptible (driver, D) clinical H pylori isolates were selected. Genomic DNAs were prepared and submitted to Rsa I digestion. Then two different adaptors were ligated respectively to the 5'-end of two aliquots of the tester DNA fragments and SSH was made between the tester and driver DNAs. The specific inserts of tester strains were screened and MTZ-resistance related gene fragments were identified by dot blotting.

Results

Among the randomly selected 120 subtractive colonies, 37 DNA fragments had a different number of DNA copies (> or = 2 times) in resistant and susceptible strains and

SUBMITTER: Li AQ 

PROVIDER: S-EPMC4149965 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature