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COP1 jointly modulates cytoskeletal processes and electrophysiological responses required for stomatal closure.

ABSTRACT: Reorganization of the cortical microtubule cytoskeleton is critical for guard cell function. Here, we investigate how environmental and hormonal signals cause these rearrangements and find that COP1, a RING-finger-type ubiquitin E3 ligase, is required for degradation of tubulin, likely by the 26S proteasome. This degradation is required for stomatal closing. In addition to regulating the cytoskeleton, we show that cop1 mutation impaired the activity of S-type anion channels, which are critical for stomatal closure. Thus, COP1 is revealed as a potential coordinator of cytoskeletal and electrophysiological activities required for guard cell function.

SUBMITTER: Khanna R

PROVIDER: S-EPMC4153439 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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COP1 jointly modulates cytoskeletal processes and electrophysiological responses required for stomatal closure.

Khanna Rajnish R Li Junlin J Tseng Tong-Seung TS Schroeder Julian I JI Ehrhardt David W DW Briggs Winslow R WR

Molecular plant 20140523 9

Reorganization of the cortical microtubule cytoskeleton is critical for guard cell function. Here, we investigate how environmental and hormonal signals cause these rearrangements and find that COP1, a RING-finger-type ubiquitin E3 ligase, is required for degradation of tubulin, likely by the 26S proteasome. This degradation is required for stomatal closing. In addition to regulating the cytoskeleton, we show that cop1 mutation impaired the activity of S-type anion channels, which are critical f ...[more]

PMID: 25151660

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