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The xenograft antigen in complex with GS-1-B4 lectin: crystallization and preliminary X-ray analysis.

ABSTRACT: The implantation of animal organs is one approach to overcoming the shortage of human donor organs for medical transplantation. Although readily available, non-primate tissues are subject to hyperacute rejection wherein human anti-Galalpha(1-3)Gal antibodies react with haptens present on the transplanted cells' surfaces. The understanding of this interaction on a molecular level will further the development of a strategy for the prevention of hyperacute rejection in xenotransplantation. The Galalpha(1-3)Gal hapten ('xenograft antigen') has been cocrystallized with the Gal-specific B(4) isolectin of Griffonia simplicifolia lectin-1. Crystals were analyzed by cryocrystallography and were found to diffract to moderately high resolution on a rotating-anode X-ray source. They belong to the P2(1)2(1)2 space group, with unit-cell parameters a = 111.0, b = 51.3, c = 76.9 A, and contain two molecules per asymmetric unit.

SUBMITTER: Tempel W

PROVIDER: S-EPMC4190837 | biostudies-literature | 2001 Nov

REPOSITORIES: biostudies-literature

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The xenograft antigen in complex with GS-1-B4 lectin: crystallization and preliminary X-ray analysis.

Tempel W W Lipscomb L A LA Rose J P JP Woods R J RJ

Acta crystallographica. Section D, Biological crystallography 20011025 Pt 11

The implantation of animal organs is one approach to overcoming the shortage of human donor organs for medical transplantation. Although readily available, non-primate tissues are subject to hyperacute rejection wherein human anti-Galalpha(1-3)Gal antibodies react with haptens present on the transplanted cells' surfaces. The understanding of this interaction on a molecular level will further the development of a strategy for the prevention of hyperacute rejection in xenotransplantation. The Gala ...[more]

PMID: 11679730

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