CREB mediates the insulinotropic and anti-apoptotic effects of GLP-1 signaling in adult mouse β-cells.

ABSTRACT:

Objective

Glucagon-like peptide-1 (GLP-1) plays a major role in pancreatic β-cell function and survival by increasing cytoplasmic cAMP levels, which are thought to affect transcription through activation of the basic leucine zipper (bZIP) transcription factor CREB. Here, we test CREB function in the adult β-cell through inducible gene deletion.

Methods

We employed cell type-specific and inducible gene ablation to determine CREB function in pancreatic β-cells in mice.

Results

By ablating CREB acutely in mature β-cells in tamoxifen-treated Creb (loxP/loxP);Pdx1-CreERT2 mice, we show that CREB has little impact on β-cell turnover, in contrast to what had been postulated previously. Rather, CREB is required for GLP-1 to elicit its full effects on stimulating glucose-induc

SUBMITTER: Shin S 

PROVIDER: S-EPMC4216406 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature