Stability of the CpG island methylator phenotype during glioma progression and identification of methylated loci in secondary glioblastomas.

ABSTRACT:

Background

Grade IV glioblastomas exist in two forms, primary (de novo) glioblastomas (pGBM) that arise without precursor lesions, and the less common secondary glioblastomas (sGBM) which develop from earlier lower grade lesions. Genetic heterogeneity between pGBM and sGBM has been documented as have differences in the methylation of individual genes. A hypermethylator phenotype in grade IV GBMs is now well documented however there has been little comparison between global methylation profiles of pGBM and sGBM samples or of methylation profiles between paired early and late sGBM samples.

Methods

We performed genome-wide methylation profiling of 20 matched pairs of early and late gliomas using the Infinium HumanMethylation450 BeadChips to assess methylation at >485,000 cytosi

SUBMITTER: Hill VK 

PROVIDER: S-EPMC4227105 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature