RNA-seq analysis of the influence of anaerobiosis and FNR on Shigella flexneri.

ABSTRACT:

Background

Shigella flexneri is an important human pathogen that has to adapt to the anaerobic environment in the gastrointestinal tract to cause dysentery. To define the influence of anaerobiosis on the virulence of Shigella, we performed deep RNA sequencing to identify transcriptomic differences that are induced by anaerobiosis and modulated by the anaerobic Fumarate and Nitrate Reduction regulator, FNR.

Results

We found that 528 chromosomal genes were differentially expressed in response to anaerobic conditions; of these, 228 genes were also influenced by FNR. Genes that were up-regulated in anaerobic conditions are involved in carbon transport and metabolism (e.g. ptsG, manX, murQ, cysP, cra), DNA topology and regulation (e.g. ygiP, stpA, hns), host interactions (e.g. yc

SUBMITTER: Vergara-Irigaray M 

PROVIDER: S-EPMC4229854 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature