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A spatially and chemically defined platform for the uniform growth of human pluripotent stem cells.

ABSTRACT: We report the design of a chemically defined platform engineered for the culture of human pluripotent stem cells (hPSCs) that supports the long-term maintenance of self-renewing hPSC populations in a more uniform manner than standard culture systems. Microcontact printing (?CP) of alkanethiol self-assembled monolayers (SAMs) was used to spatially direct hPSC adherence. This technique not only establishes control over hPSC colony size and shape but also preserves genetic stability and provides unprecedented uniformity in the pluripotency of hPSC populations that is quantitatively assessed in the present study.

SUBMITTER: Jonas SJ 

PROVIDER: S-EPMC4247494 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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A spatially and chemically defined platform for the uniform growth of human pluripotent stem cells.

Jonas Steven J SJ   Alva Jackelyn A JA   Richardson Wade W   Sherman Sean P SP   Galic Zoran Z   Pyle April D AD   Dunn Bruce B  

Materials science & engineering. C, Materials for biological applications 20120911 1

We report the design of a chemically defined platform engineered for the culture of human pluripotent stem cells (hPSCs) that supports the long-term maintenance of self-renewing hPSC populations in a more uniform manner than standard culture systems. Microcontact printing (μCP) of alkanethiol self-assembled monolayers (SAMs) was used to spatially direct hPSC adherence. This technique not only establishes control over hPSC colony size and shape but also preserves genetic stability and provides un  ...[more]

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