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Comparison of test specificities of commercial antigen-based assays and in-house PCR methods for detection of rotavirus in stool specimens.

ABSTRACT: Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.

SUBMITTER: Ye S

PROVIDER: S-EPMC4290940 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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Comparison of test specificities of commercial antigen-based assays and in-house PCR methods for detection of rotavirus in stool specimens.

Ye S S Lambert S B SB Grimwood K K Roczo-Farkas S S Nimmo G R GR Sloots T P TP Kirkwood C D CD Whiley D M DM

Journal of clinical microbiology 20141022 1

Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection. ...[more]

PMID: 25339400

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