Integrative genomics and transcriptomics analysis of human embryonic and induced pluripotent stem cells.

ABSTRACT:

Background

Human genomic variations, including single nucleotide polymorphisms (SNPs) and copy number variations (CNVs), are associated with several phenotypic traits varying from mild features to hereditary diseases. Several genome-wide studies have reported genomic variants that correlate with gene expression levels in various tissue and cell types.

Results

We studied human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) measuring the SNPs and CNVs with Affymetrix SNP 6 microarrays and expression values with Affymetrix Exon microarrays. We computed the linear relationships between SNPs and expression levels of exons, transcripts and genes, and the associations between gene CNVs and gene expression levels. Further, for a few of the resulted ge

SUBMITTER: Laurila K 

PROVIDER: S-EPMC4298950 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature