Isolation, Identification, and Pathogenicity of Neospora caninum China Yanbian strain.

ABSTRACT:

Background

The aim of the study was to provide a point of reference to study the Neospora caninum infections in China.

Methods

Genome DNA was extracted from the brains of aborted fetuses and specific PCR was performed with N. caninum Nc5-targeted specific primers. Fetal bovine brain tissues were homogenized and continuously cultured in Vero cells with double antibodies. The medium was replaced at 2-d intervals and the state of cells was observed.

Results

A 608 bp Nc5 gene band was detected by PCR amplification. After sequencing, the sequence of the sample shared 99.5% homology with GenBank (AF061249). Brain homogenates were continuously cultured in Vero cells for 34 d and N. caninum was found. The results of IFAT and Nc5 gene-based PCR detection were N. caninum-positi

SUBMITTER: Jia LJ 

PROVIDER: S-EPMC4316571 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature