Cloning and expression of the enzymatic region of Streptococcal hyaluronidase.

ABSTRACT:

Objectives

Streptococcus pyogenes produces extracellular hyaluronidase enzyme. This enzyme is directly associated with the spread of the organism during infection. The objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity.

Materials and methods

The enzymatic region of hyaluronidase gene was detected by bioinformatics method. The PCR method was used to amplify enzymatic region of hyaluronidase gene from chromosomal DNA of Streptococcus pyogenes. The eluted product was cloned into the prokaryotic expression vector pET32a which was digested by BamHI and HindIII restriction endonuclease enzymes. The target protein was expressed in the Escherichia coli. The bacteria including pET32a-hylA

SUBMITTER: Mirjamali NA 

PROVIDER: S-EPMC4322150 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature