Checkpoints are blind to replication restart and recombination intermediates that result in gross chromosomal rearrangements.

ABSTRACT: Replication fork inactivation can be overcome by homologous recombination, but this can cause gross chromosomal rearrangements that subsequently missegregate at mitosis, driving further chromosome instability. It is unclear when the chromosome rearrangements are generated and whether individual replication problems or the resulting recombination intermediates delay the cell cycle. Here we have investigated checkpoint activation during HR-dependent replication restart using a site-specific replication fork-arrest system. Analysis during a single cell cycle shows that HR-dependent replication intermediates arise in S phase, shortly after replication arrest, and are resolved into acentric and dicentric chromosomes in G2. Despite this, cells progress into mitosis without delay. Neither the DNA

SUBMITTER: Mohebi S 

PROVIDER: S-EPMC4351560 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature