Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction.

ABSTRACT: Structured illumination microscopy (SIM) with axially optical sectioning capability has found widespread applications in three-dimensional live cell imaging in recent years, since it combines high sensitivity, short image acquisition time, and high spatial resolution. To obtain one sectioned slice, three raw images with a fixed phase-shift, normally 2π/3, are generally required. In this paper, we report a data processing algorithm based on the one-dimensional Hilbert transform, which needs only two raw images with arbitrary phase-shift for each single slice. The proposed algorithm is different from the previous two-dimensional Hilbert spiral transform algorithm in theory. The presented algorithm has the advantages of simpler data processing procedure, faster computation speed and better re

SUBMITTER: Zhou X 

PROVIDER: S-EPMC4370656 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature