The influence of human GSTZ1 gene haplotype variations on GSTZ1 expression.

ABSTRACT:

Background/objectives

The zeta-1 family isoform of GST biotransforms the investigational drug dichloroacetate (DCA) and certain other halogenated carboxylic acids. Haplotype variability in GSTZ1 influences the kinetics and, possibly, the toxicity of DCA. DCA metabolism correlates with expression of the GSTZ1 protein, so it is important to document variables that affect expression. Following up on a limited previous study, we tested the hypothesis that a coding single nucleotide polymorphism (SNP), the lysine (K) amino acid (E32>K) in GSTZ1 haplotypes linked to a promoter region SNP results in lower hepatic expression of GSTZ1.

Materials and methods

The influence of K carrier and non-K carrier haplotypes on GSTZ1 expression was determined by analyzing 78 liver samples from in

SUBMITTER: Langaee TY 

PROVIDER: S-EPMC4382440 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature