Unknown

Dataset Information

0

Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats.


ABSTRACT: We examined the molecular and metabolomic effects of voluntary running wheel activity in late middle-aged male Sprague Dawley rats (16-17 months). Rats were assigned either continuous voluntary running wheel access for 8 weeks (RW+) or cage-matched without running wheel access (RW-). The 9 RW+ rats averaged 83 m/day (range: 8-163 m), yet exhibited both 84% reduced individual body weight gain (4.3 g vs. 26.3 g, P = 0.02) and 6.5% reduced individual average daily food intake (20.6 g vs. 22.0 g, P = 0.09) over the 8 weeks. Hindlimb muscles were harvested following an overnight fast. Muscle weights and myofiber cross-sectional area showed no difference between groups. Western blots of gastrocnemius muscle lysates with a panel of antibodies suggest that running wheel activity improved oxidative metabolism (53% increase in PGC1?, P = 0.03), increased autophagy (36% increase in LC3B-II/-I ratio, P = 0.03), and modulated growth signaling (26% increase in myostatin, P = 0.04). RW+ muscle also showed 43% increased glycogen phosphorylase expression (P = 0.04) and 45% increased glycogen content (P = 0.04). Metabolomic profiling of plantaris and soleus muscles indicated that even low-volume voluntary running wheel activity is associated with decreases in many long-chain fatty acids (e.g., palmitoleate, myristoleate, and eicosatrienoate) relative to RW- rats. Relative increases in acylcarnitines and acyl glycerophospholipids were also observed in RW+ plantaris. These data establish that even modest amounts of physical activity during late middle-age promote extensive metabolic remodeling of skeletal muscle.

SUBMITTER: Garvey SM 

PROVIDER: S-EPMC4393218 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats.

Garvey Sean M SM   Russ David W DW   Skelding Mary B MB   Dugle Janis E JE   Edens Neile K NK  

Physiological reports 20150225 2


We examined the molecular and metabolomic effects of voluntary running wheel activity in late middle-aged male Sprague Dawley rats (16-17 months). Rats were assigned either continuous voluntary running wheel access for 8 weeks (RW+) or cage-matched without running wheel access (RW-). The 9 RW+ rats averaged 83 m/day (range: 8-163 m), yet exhibited both 84% reduced individual body weight gain (4.3 g vs. 26.3 g, P = 0.02) and 6.5% reduced individual average daily food intake (20.6 g vs. 22.0 g, P  ...[more]

Similar Datasets

| S-EPMC7745540 | biostudies-literature
| S-EPMC4988888 | biostudies-literature
| S-EPMC8020351 | biostudies-literature
| S-EPMC4238425 | biostudies-literature
| S-EPMC6301263 | biostudies-literature
| S-EPMC6639307 | biostudies-literature
| S-EPMC4019835 | biostudies-literature
| S-EPMC2721000 | biostudies-literature
| S-EPMC3152565 | biostudies-literature
| S-EPMC8280765 | biostudies-literature