Capture compound mass spectrometry--a powerful tool to identify novel c-di-GMP effector proteins.

ABSTRACT: Considerable progress has been made during the last decade towards the identification and characterization of enzymes involved in the synthesis (diguanylate cyclases) and degradation (phosphodiesterases) of the second messenger c-di-GMP. In contrast, little information is available regarding the molecular mechanisms and cellular components through which this signaling molecule regulates a diverse range of cellular processes. Most of the known effector proteins belong to the PilZ family or are degenerated diguanylate cyclases or phosphodiesterases that have given up on catalysis and have adopted effector function. Thus, to better define the cellular c-di-GMP network in a wide range of bacteria experimental methods are required to identify and validate novel effectors for which reliable in s

SUBMITTER: Laventie BJ 

PROVIDER: S-EPMC4401386 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature