Identification of genes associated with resilience/vulnerability to sleep deprivation and starvation in Drosophila.

ABSTRACT:

Background and study objectives

Flies mutant for the canonical clock protein cycle (cyc(01)) exhibit a sleep rebound that is ∼10 times larger than wild-type flies and die after only 10 h of sleep deprivation. Surprisingly, when starved, cyc(01) mutants can remain awake for 28 h without demonstrating negative outcomes. Thus, we hypothesized that identifying transcripts that are differentially regulated between waking induced by sleep deprivation and waking induced by starvation would identify genes that underlie the deleterious effects of sleep deprivation and/or protect flies from the negative consequences of waking.

Design

We used partial complementary DNA microarrays to identify transcripts that are differentially expressed between cyc(01) mutants that had been sleep depri

SUBMITTER: Thimgan MS 

PROVIDER: S-EPMC4402663 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature