Online matrix removal platform for coupling gel-based separations to whole protein electrospray ionization mass spectrometry.

ABSTRACT: A fractionation method called gel-eluted liquid fraction entrapment electrophoresis (GELFrEE) has been used to dramatically increase the number of proteins identified in top-down proteomic workflows; however, the technique involves the use of sodium dodecyl sulfate (SDS), a surfactant that interferes with electrospray ionization. Therefore, an efficient removal of SDS is absolutely required prior to mass analysis. Traditionally, methanol/chloroform precipitation and spin columns have been used, but they lack reproducibility and are difficult to automate. Therefore, we developed an in-line matrix removal platform to enable the direct analysis of samples containing SDS and salts. Only small molecules like SDS permeate a porous membrane and are removed in a manner similar to cross-flow filtra

SUBMITTER: Kim KH 

PROVIDER: S-EPMC4419745 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature