Ontology highlight
ABSTRACT:
SUBMITTER: Yamamoto K
PROVIDER: S-EPMC4434770 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Yamamoto Kenta K Kishida Tsunao T Sato Yoshiki Y Nishioka Keisuke K Ejima Akika A Fujiwara Hiroyoshi H Kubo Toshikazu T Yamamoto Toshiro T Kanamura Narisato N Mazda Osam O
Proceedings of the National Academy of Sciences of the United States of America 20150427 19
Osteoblasts produce calcified bone matrix and contribute to bone formation and remodeling. In this study, we established a procedure to directly convert human fibroblasts into osteoblasts by transducing some defined factors and culturing in osteogenic medium. Osteoblast-specific transcription factors, Runt-related transcription factor 2 (Runx2), and Osterix, in combination with Octamer-binding transcription factor 3/4 (Oct4) and L-Myc (RXOL) transduction, converted ∼ 80% of the fibroblasts into ...[more]