Dataset Information
Multilayered HIV-1 gag-specific T-cell responses contribute to slow progression in HLA-A*30-B*13-C*06-positive patients.
Ontology highlight
ABSTRACT: Objective
The HLA-A30-B13-C06 haplotype is reported to be associated with slow disease progression in the HIV-1-infected Northern Han Chinese population, but the mechanism remains unknown.Design
Gag-specific T-cell responses and gag sequencing were performed in nine B' clade HIV-1-infected HLA-A30-B13-C06-positive slow progressors to understand HLA-associated viral control.Methods
Interferon-γ ELISPOT assays were performed to determine the Gag-specific T-cell responses and cross-reactivity to variant peptides. Longitudinal HIV-1 gag sequencing was performed at the clonal level.Results
The overlapping peptides (OLP)-48: RQANFLGKIWPSHKGRPGNF (RL42 Gag434-453); OLP-2: GQLDRWEKIRLRPGGKKKYR (RL42 Gag11-30); OLP-15: VQNLQGQMVHQPISPRTLNA (RL42 Gag135-154) and OLP-16: HQPISPRTLNAWVKVVEEKA (RL42 Gag144-163) were dominant in HLA-A30-B13-C06-positive patients. A new epitope [HQPISPRTL (Gag144-152, HL9)] within OLP-15 and OLP-16 was identified. Results showed that strong cross-reactive responses to multiple immunodominant peptides were associated with better clinical outcomes. In addition, efficient cross-recognition of HL9 autologous variants developed in patients was associated with high CD4 T-cell counts. However, two patients who had developed mutations to their dominant responses during the follow-up experienced decrease in CD4 T-cell counts. It appears that Gag-specific T-cell responses against one or more unmutated epitopes or cross-recognition of autologous epitope variants contribute to slow disease progression in HLA-A30-B13-C06-positive patients.Conclusion
We conclude that a single 'appropriate' Gag-specific T-cell response appears to be sufficient to protect patients from disease progression. HLA-A30-B13-C06-positive individuals benefited from having a choice of numerous immunodominant gag epitopes for T cells to react. The study offers new insight for future design of T-cell-based HIV-1 vaccine.
SUBMITTER: Zhang H
PROVIDER: S-EPMC4444423 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Publications
Multilayered HIV-1 gag-specific T-cell responses contribute to slow progression in HLA-A*30-B*13-C*06-positive patients.
AIDS (London, England) 20150601 9
<h4>Objective</h4>The HLA-A30-B13-C06 haplotype is reported to be associated with slow disease progression in the HIV-1-infected Northern Han Chinese population, but the mechanism remains unknown.<h4>Design</h4>Gag-specific T-cell responses and gag sequencing were performed in nine B' clade HIV-1-infected HLA-A30-B13-C06-positive slow progressors to understand HLA-associated viral control.<h4>Methods</h4>Interferon-γ ELISPOT assays were performed to determine the Gag-specific T-cell responses an ...[more]