Multilayered HIV-1 gag-specific T-cell responses contribute to slow progression in HLA-A*30-B*13-C*06-positive patients.

ABSTRACT:

Objective

The HLA-A30-B13-C06 haplotype is reported to be associated with slow disease progression in the HIV-1-infected Northern Han Chinese population, but the mechanism remains unknown.

Design

Gag-specific T-cell responses and gag sequencing were performed in nine B' clade HIV-1-infected HLA-A30-B13-C06-positive slow progressors to understand HLA-associated viral control.

Methods

Interferon-γ ELISPOT assays were performed to determine the Gag-specific T-cell responses and cross-reactivity to variant peptides. Longitudinal HIV-1 gag sequencing was performed at the clonal level.

Results

The overlapping peptides (OLP)-48: RQANFLGKIWPSHKGRPGNF (RL42 Gag434-453); OLP-2: GQLDRWEKIRLRPGGKKKYR (RL42 Gag11-30); OLP-15: VQNLQGQMVHQPISPRTLNA (RL42 Gag135-154) and OLP-1

SUBMITTER: Zhang H 

PROVIDER: S-EPMC4444423 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature