Relationship between insecticide resistance and kdr mutations in the dengue vector Aedes aegypti in Southern China.

ABSTRACT:

Background

Aedes aegypti is an important vector for dengue virus and thus has been targeted with pyrethroid insecticides in many areas of the world. As such, resistance has been detected to several of these insecticides, including in China, but the mechanisms of the resistance are not well understood in this country.

Methods

Using the World Health Organization larval mosquito bioassay, five field populations of Aedes aegypti from Southern China were characterized for their resistance to cypermethrin and cyhalothrin. RNA extraction with PCR amplification, cloning and sequencing of the sodium channel gene was followed by comparisons of susceptible and wild mosquito strains Additionally, genomic DNA was used for Allele-specific PCR (AS-PCR) genotyping of the sodium channel gene

SUBMITTER: Li CX 

PROVIDER: S-EPMC4475621 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature