In Vitro Differentiation of Human Neural Progenitor Cells Into Striatal GABAergic Neurons.

ABSTRACT:

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: Huntington's disease (HD) results from a CAG repeat expansion in the gene encoding the huntingtin protein. This inherited disorder is characterized by progressive neurodegeneration. In particular, HD progression involves the loss of striatal projection neurons. The limited availability of reliable sources of human striatal projection neurons currently hampers our understanding of HD mechanisms and hinders the development of novel HD treatments. In this paper, we described two- and three-step methods for differentiating human neural progenitor cells toward striatal projection neurons. In the two-step differentiation protocol, 90%, 54%, and 6% of MAP2-positive cells were immunopositive for GABA, calbindin (CALB1), and DARPP-32/PPP1R1B, respectively. In the three-step dif

SUBMITTER: Lin L 

PROVIDER: S-EPMC4479615 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature