Identification and visualization of multidimensional antigen-specific T-cell populations in polychromatic cytometry data.

ABSTRACT: An important aspect of immune monitoring for vaccine development, clinical trials, and research is the detection, measurement, and comparison of antigen-specific T-cells from subject samples under different conditions. Antigen-specific T-cells compose a very small fraction of total T-cells. Developments in cytometry technology over the past five years have enabled the measurement of single-cells in a multivariate and high-throughput manner. This growth in both dimensionality and quantity of data continues to pose a challenge for effective identification and visualization of rare cell subsets, such as antigen-specific T-cells. Dimension reduction and feature extraction play pivotal role in both identifying and visualizing cell populations of interest in large, multi-dimensional cytometry da

SUBMITTER: Lin L 

PROVIDER: S-EPMC4482785 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature