Antagonistic peptide technology for functional dissection of CLE peptides revisited.

ABSTRACT: In the Arabidopsis thaliana genome, over 1000 putative genes encoding small, presumably secreted, signalling peptides can be recognized. However, a major obstacle in identifying the function of genes encoding small signalling peptides is the limited number of available loss-of-function mutants. To overcome this, a promising new tool, antagonistic peptide technology, was recently developed. Here, this antagonistic peptide technology was tested on selected CLE peptides and the related IDA peptide and its usefulness in the context of studies of peptide function discussed. Based on the analyses, it was concluded that the antagonistic peptide approach is not the ultimate means to overcome redundancy or lack of loss-of-function lines. However, information collected using antagonistic peptide app

SUBMITTER: Czyzewicz N 

PROVIDER: S-EPMC4526918 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature