Ontology highlight
ABSTRACT:
SUBMITTER: Katic I
PROVIDER: S-EPMC4528321 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
G3 (Bethesda, Md.) 20150603 8
Precise genome editing by the Cas9 nuclease depends on exogenously provided templates for homologous recombination. Here, we compare oligonucleotides with short homology and circular DNA molecules with extensive homology to genomic targets as templates for homology-based repair of CRISPR/Cas9 induced double-strand breaks. We find oligonucleotides to be templates of choice for introducing small sequence changes into the genome based on editing efficiency and ease of use. We show that polarity of ...[more]