Dataset Information

Gene expression profile in liver of hB1F transgenic mice.

ABSTRACT:

Aim

To analyze the tissue morphologic phenotype and liver gene expression profile of hB1F transgenic mice.

Methods

Transgene expression was analyzed with RT-PCR and Western blotting. For one of the transgenic mouse lines, tissue expression pattern of the transgene was also examined with immunochemical methods. Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice. The liver gene expression profile of transgenic mice was analyzed with microchip, and some of the differentially expressed genes were verified with RT-PCR.

Results

The expressions of hB1F were shown in livers from 6 of 7 transgenic mouse lines. The overexpression of hB1F transgene did not cause pathological changes. Expressions of three genes were up-regulated, while down-regulation was observed for 25 genes.

Conclusion

The overexpression of hB1F transgene may cause changes of gene expression profiles in the liver of transgenic mice.

SUBMITTER: Wang SL

PROVIDER: S-EPMC4576262 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature

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Publications

Gene expression profile in liver of hB1F transgenic mice.

Wang Shui-Liang SL Yang Hua H Xie You-Hua YH Wang Yuan Y Li Jian-Zhong JZ Wang Long L Wang Zhu-Gang ZG Fu Ji-Liang JL

World journal of gastroenterology 20041001 20

<h4>Aim</h4>To analyze the tissue morphologic phenotype and liver gene expression profile of hB1F transgenic mice.<h4>Methods</h4>Transgene expression was analyzed with RT-PCR and Western blotting. For one of the transgenic mouse lines, tissue expression pattern of the transgene was also examined with immunochemical methods. Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice. The liver gene expression profile of transgenic mice was analyzed ...[more]

PMID: 15378783

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Dataset Information

Gene expression profile in liver of hB1F transgenic mice.

Aim

Methods

Results

Conclusion

Publications

Gene expression profile in liver of hB1F transgenic mice.

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