ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors.

ABSTRACT: Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase ('tagmentation'). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.

SUBMITTER: Schmidl C 

PROVIDER: S-EPMC4589892 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature