Signal strength regulates antigen-mediated T-cell deceleration by distinct mechanisms to promote local exploration or arrest.

ABSTRACT: T lymphocytes are highly motile cells that decelerate upon antigen recognition. These cells can either completely stop or maintain a low level of motility, forming contacts referred to as synapses or kinapses, respectively. Whether similar or distinct molecular mechanisms regulate T-cell deceleration during synapses or kinapses is unclear. Here, we used microfabricated channels and intravital imaging to observe and manipulate T-cell kinapses and synapses. We report that high-affinity antigen induced a pronounced deceleration selectively dependent on Ca(2+) signals and actin-related protein 2/3 complex (Arp2/3) activity. In contrast, low-affinity antigens induced a switch of migration mode that promotes T-cell exploratory behavior, characterized by partial deceleration and frequent directio

SUBMITTER: Moreau HD 

PROVIDER: S-EPMC4593123 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature