Identification of novel sRNAs involved in biofilm formation, motility, and fimbriae formation in Escherichia coli.

ABSTRACT: Bacterial small RNAs (sRNAs) are known regulators in many physiological processes. In Escherichia coli, a large number of sRNAs have been predicted, among which only about a hundred are experimentally validated. Despite considerable research, the majority of their functions remain uncovered. Therefore, collective analysis of the roles of sRNAs in specific cellular processes may provide an effective approach to identify their functions. Here, we constructed a collection of plasmids overexpressing 99 individual sRNAs, and analyzed their effects on biofilm formation and related phenotypes. Thirty-three sRNAs significantly affecting these cellular processes were identified. No consistent correlations were observed, except that all five sRNAs suppressing type I fimbriae inhibited biofilm format

SUBMITTER: Bak G 

PROVIDER: S-EPMC4606813 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature