Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea.

ABSTRACT: The basidiomycete fungus Coprinopsis cinerea is an important model system for multicellular development. Fruiting bodies of C. cinerea are typical mushrooms, which can be produced synchronously on defined media in the laboratory. To investigate the transcriptome in detail during fruiting body development, high-throughput sequencing (RNA-seq) was performed using cDNA libraries strand-specifically constructed from 13 points (stages/tissues) with two biological replicates. The reads were aligned to 14,245 predicted transcripts, and counted for forward and reverse transcripts. Differentially expressed genes (DEGs) between two adjacent points and between vegetative mycelium and each point were detected by Tag Count Comparison (TCC). To validate RNA-seq data, expression levels of selected genes

SUBMITTER: Muraguchi H 

PROVIDER: S-EPMC4624876 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature