Recruitment of scribble to the synaptic scaffolding complex requires GUK-holder, a novel DLG binding protein.

ABSTRACT:

Background

Membrane-associated guanylate kinases (MAGUKs), such as Discs-Large (DLG), play critical roles in synapse maturation by regulating the assembly of synaptic multiprotein complexes. Previous studies have revealed a genetic interaction between DLG and another PDZ scaffolding protein, SCRIBBLE (SCRIB), during the establishment of cell polarity in developing epithelia. A possible interaction between DLG and SCRIB at synaptic junctions has not yet been addressed. Likewise, the biochemical nature of this interaction remains elusive, raising questions regarding the mechanisms by which the actions of both proteins are coordinated.

Results

Here we report the isolation of a new DLG-interacting protein, GUK-holder, that interacts with the GUK domain of DLG and which is dynami

SUBMITTER: Mathew D 

PROVIDER: S-EPMC4661175 | biostudies-literature | 2002 Apr

REPOSITORIES: biostudies-literature