Novel green tissue-specific synthetic promoters and cis-regulatory elements in rice.

ABSTRACT: As an important part of synthetic biology, synthetic promoter has gradually become a hotspot in current biology. The purposes of the present study were to synthesize green tissue-specific promoters and to discover green tissue-specific cis-elements. We first assembled several regulatory sequences related to tissue-specific expression in different combinations, aiming to obtain novel green tissue-specific synthetic promoters. GUS assays of the transgenic plants indicated 5 synthetic promoters showed green tissue-specific expression patterns and different expression efficiencies in various tissues. Subsequently, we scanned and counted the cis-elements in different tissue-specific promoters based on the plant cis-elements database PLACE and the rice cDNA microarray database CREP for green tis

SUBMITTER: Wang R 

PROVIDER: S-EPMC4676006 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature