NanoOK: multi-reference alignment analysis of nanopore sequencing data, quality and error profiles.

ABSTRACT:

Motivation

The Oxford Nanopore MinION sequencer, currently in pre-release testing through the MinION Access Programme (MAP), promises long reads in real-time from an inexpensive, compact, USB device. Tools have been released to extract FASTA/Q from the MinION base calling output and to provide basic yield statistics. However, no single tool yet exists to provide comprehensive alignment-based quality control and error profile analysis--something that is extremely important given the speed with which the platform is evolving.

Results

NanoOK generates detailed tabular and graphical output plus an in-depth multi-page PDF report including error profile, quality and yield data. NanoOK is multi-reference, enabling detailed analysis of metagenomic or multiplexed samples. Four popula

SUBMITTER: Leggett RM 

PROVIDER: S-EPMC4681994 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature