Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay.

ABSTRACT: T-cells are remarkably specific and effective when recognizing antigens in the form of peptides embedded in MHC molecules (pMHC) on the surface of Antigen Presenting Cells (APCs). This is despite T-cell antigen receptors (TCRs) exerting usually a moderate affinity (µM range) to antigen when binding is measured in vitro(1). In view of the molecular and cellular parameters contributing to T-cell antigen sensitivity, a microscopy-based methodology has been developed as a means to monitor TCR-pMHC binding in situ, as it occurs within the synapse of a live T-cell and an artificial and functionalized glass-supported planar lipid bilayer (SLB), which mimics the cell membrane of an Antigen presenting Cell (APC) (2). Measurements are based on Förster Resonance Energy Transfer (FRET) between a blue-

SUBMITTER: Axmann M 

PROVIDER: S-EPMC4692690 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature