Concurrent measures of total and integrated HIV DNA monitor reservoirs and ongoing replication in eradication trials.

ABSTRACT:

Objectives

Interest in targeting HIV reservoirs is fueling trials that may decrease reservoir size and/or induce viral replication. Therefore, we aimed to develop strategies to sensitively measure changes in these parameters in patients on and off antiretroviral therapy (ART). Achieving these goals may help evaluate the effects of future clinical trials.

Design

To determine the relationship between measurements of total and integrated HIV DNA and their role as markers of reservoir size and ongoing replication, these parameters were measured during the first year of ART, during long-term effective ART, and during a clinical trial aimed at targeting reservoirs.

Methods

Total and integrated HIV DNA were measured in patient samples using quantitative PCR techniques. CD4(+

SUBMITTER: Mexas AM 

PROVIDER: S-EPMC4692807 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature