An approach to improve the resolution of helical filaments with a large axial rise and flexible subunits.

ABSTRACT: Single particle analysis is widely used for three-dimensional reconstruction of helical filaments. Near-atomic resolution has been obtained for several well-ordered filaments. However, it is still a challenge to achieve high resolution for filaments with flexible subunits and a large axial rise per subunit relative to pixel size. Here, we describe an approach that improves the resolution in such cases. In filaments with a large axial rise, many segments must be shifted a long distance along the filament axis to match with a reference projection, potentially causing loss of alignment accuracy and hence resolution. In our study of myosin filaments, we overcame this problem by pre-determining the axial positions of myosin head crowns within segments to decrease the alignment error. In additio

SUBMITTER: Yang S 

PROVIDER: S-EPMC4696882 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature