Ontology highlight
ABSTRACT:
SUBMITTER: Yang Z
PROVIDER: S-EPMC4712191 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Molecular cell 20151206 1
PIWI-interacting RNAs (piRNAs) guide PIWI proteins to suppress transposons in the cytoplasm and nucleus of animal germ cells, but how silencing in the two compartments is coordinated is not known. Here we demonstrate that endonucleolytic slicing of a transcript by the cytosolic mouse PIWI protein MILI acts as a trigger to initiate its further 5'→3' processing into non-overlapping fragments. These fragments accumulate as new piRNAs within both cytosolic MILI and the nuclear MIWI2. We also identif ...[more]