Single-Cell mRNA Profiling Reveals Cell-Type-Specific Expression of Neurexin Isoforms.

ABSTRACT: Neurexins are considered central organizers of synapse architecture that are implicated in neuropsychiatric disorders. Expression of neurexins in hundreds of alternatively spliced isoforms suggested that individual neurons might exhibit a cell-type-specific neurexin expression pattern (a neurexin code). To test this hypothesis, we quantified the single-cell levels of neurexin isoforms and other trans-synaptic cell-adhesion molecules by microfluidics-based RT-PCR. We show that the neurexin repertoire displays pronounced cell-type specificity that is remarkably consistent within each type of neuron. Furthermore, we uncovered region-specific regulation of neurexin transcription and splice-site usage. Finally, we demonstrate that the transcriptional profiles of neurexins can be altered in an e

SUBMITTER: Fuccillo MV 

PROVIDER: S-EPMC4733560 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature