Optimization of Recombinant Expression of Synthetic Bacterial Phytase in Pichia pastoris Using Response Surface Methodology.

ABSTRACT:

Background

Escherichia coli phytase is an acidic histidine phytase with great specific activity. Pichia pastoris is a powerful system for the heterologous expression of active and soluble proteins which can express recombinant proteins in high cell density fermenter without loss of product yield and efficiently secrete heterologous proteins into the media. Recombinant protein expression is influenced by expression conditions such as temperature, concentration of inducer, and pH. By optimization, the yield of expressed proteins can be increase. Response surface methodology (RSM) has been widely used for the optimization and studying of different parameters in biotechnological processes.

Objectives

In this study, the expression of synthetic appA gene in P. pastoris was greatly

SUBMITTER: Akbarzadeh A 

PROVIDER: S-EPMC4746705 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature