Differential gene network analysis for the identification of asthma-associated therapeutic targets in allergen-specific T-helper memory responses.

ABSTRACT:

Background

Asthma is strongly associated with allergic sensitization, but the mechanisms that determine why only a subset of atopics develop asthma are not well understood. The aim of this study was to test the hypothesis that variations in allergen-driven CD4 T cell responses are associated with susceptibility to expression of asthma symptoms.

Methods

The study population consisted of house dust mite (HDM) sensitized atopics with current asthma (n = 22), HDM-sensitized atopics without current asthma (n = 26), and HDM-nonsensitized controls (n = 24). Peripheral blood mononuclear cells from these groups were cultured in the presence or absence of HDM extract for 24 h. CD4 T cells were then isolated by immunomagnetic separation, and gene expression patterns were profiled on mi

SUBMITTER: Troy NM 

PROVIDER: S-EPMC4769846 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature