Ontology highlight
ABSTRACT:
SUBMITTER: Eser P
PROVIDER: S-EPMC4770384 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Eser Philipp P Wachutka Leonhard L Maier Kerstin C KC Demel Carina C Boroni Mariana M Iyer Srignanakshi S Cramer Patrick P Gagneur Julien J
Molecular systems biology 20160216 2
To decrypt the regulatory code of the genome, sequence elements must be defined that determine the kinetics of RNA metabolism and thus gene expression. Here, we attempt such decryption in an eukaryotic model organism, the fission yeast S. pombe. We first derive an improved genome annotation that redefines borders of 36% of expressed mRNAs and adds 487 non-coding RNAs (ncRNAs). We then combine RNA labeling in vivo with mathematical modeling to obtain rates of RNA synthesis and degradation for 5,4 ...[more]