Nonselective Persistence of a Rickettsia conorii Extrachromosomal Plasmid during Mammalian Infection.

ABSTRACT: Scientific analysis of the genus Rickettsia is undergoing a rapid period of change with the emergence of viable genetic tools. The development of these tools for the mutagenesis of pathogenic bacteria will permit forward genetic analysis of Rickettsia pathogenesis. Despite these advances, uncertainty still remains regarding the use of plasmids to study these bacteria in in vivo mammalian models of infection, namely, the potential for virulence changes associated with the presence of extrachromosomal DNA and nonselective persistence of plasmids in mammalian models of infection. Here, we describe the transformation of Rickettsia conorii Malish 7 with the plasmid pRam18dRGA[AmTrCh]. Transformed R. conorii stably maintains this plasmid in infected cell cultures, expresses the encoded fluoresce

SUBMITTER: Riley SP 

PROVIDER: S-EPMC4771360 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature