Long noncoding RNAs expressed in human hepatic stellate cells form networks with extracellular matrix proteins.

ABSTRACT:

Background

Hepatic fibrosis is the underlying cause of cirrhosis and liver failure in nearly every form of chronic liver disease, and hepatic stellate cells (HSCs) are the primary cell type responsible for fibrosis. Long noncoding RNAs (lncRNAs) are increasingly recognized as regulators of development and disease; however, little is known about their expression in human HSCs and their function in hepatic fibrosis.

Methods

We performed RNA sequencing and ab initio assembly of RNA transcripts to define the lncRNAs expressed in human HSC myofibroblasts. We analyzed chromatin immunoprecipitation data and expression data to identify lncRNAs that were regulated by transforming growth factor beta (TGF-β) signaling, associated with super-enhancers and restricted in expression to HSC

SUBMITTER: Zhou C 

PROVIDER: S-EPMC4804564 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature