Dataset Information

Expression levels of estrogen receptor ? mRNA in peripheral blood cells are an independent biomarker for postmenopausal osteoporosis.

ABSTRACT:

Background

The up- and down-regulation of the osteoclastogenesis response depends on the estrogen/estrogen receptor (ER) signaling pathway. Previous reports have shown that the promoter hypermethylation and gene polymorphism of ER? are risks for menopausal osteoporosis. No previous study has evaluated the expression levels of ER? mRNA in menopausal osteoporosis using human subjects. We hypothesized that ER? mRNA expression may show less resistance to postmenopausal osteoporosis.

Methods

In this study, we enrolled 107 women older than 45 years without menstruation and classified them into control, osteopenia, and osteoporosis groups depending on their T-scores. The ER? mRNA levels in peripheral blood cells (PBCs) were analyzed via quantitative real-time reverse-transcription polymerase chain reaction (QRT-PCR), and estrogen in the serum was detected via ELISA.

Results

ER? mRNA levels in PBCs had a negative correlation with age and a positive correlation with estrogen and BAP in the osteopenia and osteoporosis groups, but not in the control group. Additionally, multivariate analysis showed that older age (> 55 years), and low ER? mRNA levels in PBLs (? 250.39 copies/?g DNA) were associated with an approximately 9.188-, and 31.25-fold risk of osteoporosis.

Conclusion

We conclude that ER? mRNA levels in PBLs could be used as an independent risk factor for postmenopausal osteoporosis.

General significance

Our findings suggested that ER? mRNA levels in PBLs may be more important than age and serum estrogen levels.

SUBMITTER: Chou CW

PROVIDER: S-EPMC4816160 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Publications

Expression levels of estrogen receptor α mRNA in peripheral blood cells are an independent biomarker for postmenopausal osteoporosis.

Chou Chi-Wen CW Chiang Tsay-I TI Chang I-Chang IC Huang Chung-Hung CH Cheng Ya-Wen YW

BBA clinical 20160311

<h4>Background</h4>The up- and down-regulation of the osteoclastogenesis response depends on the estrogen/estrogen receptor (ER) signaling pathway. Previous reports have shown that the promoter hypermethylation and gene polymorphism of ERα are risks for menopausal osteoporosis. No previous study has evaluated the expression levels of ERα mRNA in menopausal osteoporosis using human subjects. We hypothesized that ERα mRNA expression may show less resistance to postmenopausal osteoporosis.<h4>Metho ...[more]

PMID: 27051599

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