Dataset Information

Red fluorescent biofilm: the thick, the old, and the cariogenic.

ABSTRACT:

Background

Some dental plaque fluoresces red. The factors involved in this fluorescence are yet unknown.

Objective

The aim of this study was to assess systematically the effect of age, thickness, and cariogenicity on the extent of red fluorescence produced by in vitro microcosm biofilms.

Design

The effects of biofilm age and thickness on red fluorescence were tested in a constant depth film fermentor (CDFF) by growing biofilms of variable thicknesses that received a constant supply of defined mucin medium (DMM) and eight pulses of sucrose/day. The influence of cariogenicity on red fluorescence was tested by growing biofilm on dentin disks receiving DMM, supplemented with three or eight pulses of sucrose/day. The biofilms were analyzed at different time points after inoculation, up to 24 days. Emission spectra were measured using a fluorescence spectrophotometer (λexc405 nm) and the biofilms were photographed with a fluorescence camera. The composition of the biofilms was assessed using 454-pyrosequecing of the 16S rDNA gene.

Results

From day 7 onward, the biofilms emitted increasing intensities of red fluorescence as evidenced by the combined red fluorescence peaks. The red fluorescence intensity correlated with biofilm thickness but not in a linear way. Biofilm fluorescence also correlated with the imposed cariogenicity, evidenced by the induced dentin mineral loss. Increasing the biofilm age or increasing the sucrose pulsing frequency led to a shift in the microbial composition. These shifts in composition were accompanied by an increase in red fluorescence.

Conclusions

The current study shows that a thicker, older, or more cariogenic biofilm results in a higher intensity of red fluorescence.

SUBMITTER: Volgenant CM

PROVIDER: S-EPMC4826460 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Publications

Red fluorescent biofilm: the thick, the old, and the cariogenic.

Volgenant Catherine M C CM Hoogenkamp Michel A MA Buijs Mark J MJ Zaura Egija E Ten Cate Jacob M JM van der Veen Monique H MH

Journal of oral microbiology 20160407

<h4>Background</h4>Some dental plaque fluoresces red. The factors involved in this fluorescence are yet unknown.<h4>Objective</h4>The aim of this study was to assess systematically the effect of age, thickness, and cariogenicity on the extent of red fluorescence produced by in vitro microcosm biofilms.<h4>Design</h4>The effects of biofilm age and thickness on red fluorescence were tested in a constant depth film fermentor (CDFF) by growing biofilms of variable thicknesses that received a constan ...[more]

PMID: 27060056

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Dataset Information

Red fluorescent biofilm: the thick, the old, and the cariogenic.

Background

Objective

Design

Results

Conclusions

Publications

Red fluorescent biofilm: the thick, the old, and the cariogenic.

Similar Datasets

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