A PCR assay detects a male-specific duplicated copy of Anti-Mullerian hormone (amh) in the lingcod (Ophiodon elongatus).

ABSTRACT:

Background

Anti-Müllerian hormone (amh) or Müllerian-inhibiting substance (mis) is a member of the transforming growth factor-β family of hormones. This gene plays a key role in vertebrate male sex-determination by inhibiting the development of the Müllerian ducts, and has been shown to be the master sex-determinant in the Patagonian pejerrey.

Results

In the lingcod, Ophiodon elongatus, both males and females share one copy of amh, however we have identified a second duplicate copy that appears solely in the male individuals. We have developed a PCR-based assay targeting the TGF-β domain of amh that provides a simple method with which to sex lingcod from a small amount of tissue. An analysis across 57 individuals gave a 100% success rate in identifying the phenotypic sex.

SUBMITTER: Rondeau EB 

PROVIDER: S-EPMC4840878 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature