Dataset Information

High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke.

ABSTRACT:

Background and purpose

Approximately 30% of strokes are cryptogenic despite an exhaustive in-hospital work-up. Analysis of clot composition following endovascular treatment could provide insight into stroke etiology. T-cells already have been shown to be a major component of vulnerable atherosclerotic carotid lesions. We therefore hypothesize that T-cell content in intracranial thrombi may also be a biomarker of atherothrombotic origin.

Materials and methods

We histopathologically investigated 54 consecutive thrombi retrieved after mechanical thrombectomy in acute stroke patients. First, thrombi were classified as fibrin-dominant, erythrocyte-dominant or mixed pattern. We then performed quantitative analysis of CD3+ cells on immunohistochemically-stained thrombi and compared T-cell content between "atherothrombotic", "cardioembolism" and "other causes" stroke subtypes.

Results

Fourteen (26%) thrombi were defined as fibrin-dominant, 15 (28%) as erythrocyte-dominant, 25 (46%) as mixed. The stroke cause was defined as "atherothrombotic" in 10 (18.5%), "cardioembolism" in 25 (46.3%), and "other causes" in 19 (35.2%). Number of T-cells was significantly higher in thrombi from the "atherothrombotic" group (53.60 ± 28.78) than in the other causes (21.77 ± 18.31; p<0.0005) or the "cardioembolism" group (20.08 ± 15.66; p<0.0003).

Conclusions

The CD3+ T-cell count in intracranial thrombi was significantly higher in "atherothrombotic" origin strokes compared to all other causes. Thrombi with high content of CD3+ cells are more likely to originate from an atherosclerotic plaque.

SUBMITTER: Dargazanli C

PROVIDER: S-EPMC4859469 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Publications

High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke.

Dargazanli Cyril C Rigau Valérie V Eker Omer O Riquelme Bareiro Carlos C Machi Paolo P Gascou Grégory G Arquizan Caroline C Ayrignac Xavier X Mourand Isabelle I Corlobé Astrid A Lobotesis Kyriakos K Molinari Nicolas N Costes Valérie V Bonafé Alain A Costalat Vincent V

PloS one 20160506 5

<h4>Background and purpose</h4>Approximately 30% of strokes are cryptogenic despite an exhaustive in-hospital work-up. Analysis of clot composition following endovascular treatment could provide insight into stroke etiology. T-cells already have been shown to be a major component of vulnerable atherosclerotic carotid lesions. We therefore hypothesize that T-cell content in intracranial thrombi may also be a biomarker of atherothrombotic origin.<h4>Materials and methods</h4>We histopathologically ...[more]

PMID: 27152622

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Dataset Information

High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke.

Background and purpose

Materials and methods

Results

Conclusions

Publications

High CD3+ Cells in Intracranial Thrombi Represent a Biomarker of Atherothrombotic Stroke.

Similar Datasets

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