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Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential.


ABSTRACT: Microbial degradation of lignin releases fermentable sugars, effective utilization of which could support biofuel production from lignocellulosic biomass. In the present study, a lignin-degrading bacterium was isolated from leaf soil and identified as Burkholderia sp. based on 16S rRNA gene sequencing. This strain was named CCA53, and its lignin-degrading capability was assessed by observing its growth on medium containing alkali lignin or lignin-associated aromatic monomers as the sole carbon source. Alkali lignin and at least eight lignin-associated aromatic monomers supported growth of this strain, and the most effective utilization was observed for p-hydroxybenzene monomers. These findings indicate that Burkholderia sp. strain CCA53 has fragmentary activity for lignin degradation.

SUBMITTER: Akita H 

PROVIDER: S-EPMC4864794 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential.

Akita Hironaga H   Kimura Zen-Ichiro Z   Mohd Yusoff Mohd Zulkhairi MZ   Nakashima Nobutaka N   Hoshino Tamotsu T  

SpringerPlus 20160511


Microbial degradation of lignin releases fermentable sugars, effective utilization of which could support biofuel production from lignocellulosic biomass. In the present study, a lignin-degrading bacterium was isolated from leaf soil and identified as Burkholderia sp. based on 16S rRNA gene sequencing. This strain was named CCA53, and its lignin-degrading capability was assessed by observing its growth on medium containing alkali lignin or lignin-associated aromatic monomers as the sole carbon s  ...[more]

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