Species-level core oral bacteriome identified by 16S rRNA pyrosequencing in a healthy young Arab population.

ABSTRACT:

Background

Reports on the composition of oral bacteriome in Arabs are lacking. In addition, the majority of previous studies on other ethnic groups have been limited by low-resolution taxonomic assignment of next-generation sequencing reads. Furthermore, there has been a conflict about the existence of a 'core' bacteriome.

Objective

The objective of this study was to characterize the healthy core oral bacteriome in a young Arab population at the species level.

Methods

Oral rinse DNA samples obtained from 12 stringently selected healthy young subjects of Arab origin were pyrosequenced (454's FLX chemistry) for the bacterial 16S V1-V3 hypervariable region at an average depth of 11,500 reads. High-quality, non-chimeric reads ≥380 bp were classified to the species level u

SUBMITTER: Al-Hebshi NN 

PROVIDER: S-EPMC4871898 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature