Preparation of purified perikaryal and synaptosomal mitochondrial fractions from relatively small hypothalamic brain samples.

ABSTRACT: In order to measure the activity of neuronal mitochondria, a representative proof of neuronal processes, physiologically relevant mitochondrial samples need to be gained as simply as possible. Existing methods are, however, either for tissue samples of large size and/or homogenous microstructures only, or are not tested for mitochondrial function measurements. In the present article we describe a gradient fractionation method to isolate viable and well-coupled mitochondria from relatively heterogeneous histological microstructures such as the hypothalamus. With this new method, we are able to isolate a sufficient amount of functional mitochondria for determination of respiratory activity, in a short period of time, using affordable equipment. •Verified by electron microscopy, our method se

SUBMITTER: Kiss DS 

PROVIDER: S-EPMC4887559 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature